Medicinal plants have bioactive compounds which are used for various human disease and also an important role in wound healing. The present study is to investigate about the Antimicrobial, phytochemical and antioxidant activity of Eupatorium triplinerve Vahl. The extracts are studied against human wound and burn pathogenic bacterial stains. E. coli, Klebsiella species, Pseudomonas species, Staphylococcus aureus, Enterobacter species. From the phytochemical study is shows the presence of steroids, glycosides, tannins, phenols, saponins and flavonoids. Plant extracts compounds were separated by thin layer chromatography and identification based on Rf values. The presence of vitamin C play and important role of antioxidants.